The four packs at a glance
| Pack | Species | Variety strength | Starting microdose |
| Mexicana | P. mexicana | 2 / 5 | Half a portion (0.5 g) |
| Tampanensis | P. tampanensis | 3 / 5 | Half a portion (0.5 g) |
| Atlantis | P. atlantis | 3 / 5 | Half a portion (0.5 g) |
| Mycotrop | Not stated | 5 / 5 | A quarter portion (0.25 g) |
The most common schedules
Fadiman protocol
One microdose day, two days off, for 4–8 weeks, then a break of 2–4 weeks.
Every other day
A microdose every second day, or on two fixed days a week, always with a day off between doses.
Tolerance breaks
Tolerance builds quickly: the Trimbos Institute advises breaks of a few weeks rather than a higher dose.
What the studies say
In the largest controlled study (Szigeti et al., 2021, 191 participants), people on placebo improved as much as those who microdosed. Dutch trials with truffles (Marschall et al., 2022; Prochazkova et al., 2026) found no clear effect on mood, anxiety or creativity, apart from a slight effect on the originality of ideas. A 2026 review rates the quality of the evidence as low, and the Trimbos Institute believes that much of the perceived effect may be a placebo effect.
Reducing the risks
- Possible side effects: headache, nausea, heart complaints, anxiety and slower reactions.
- Start with the smallest amount, on a day without obligations.
- Never with lithium or an MAOI, nor with alcohol or other drugs; never stop a prescribed treatment in order to microdose.
- Not advised with bipolar disorder, a risk of psychosis, an anxiety disorder or a cardiovascular problem, nor as self-medication.
Other products in this category
This category also includes other products, such as iboga essence and sananga: each is described on its own product page. The truffle varieties are also available in 15 g and 25 g pouches in the Magic Truffles category.
Sources: Trimbos Institute (drugsinfo.nl); Unity; Microdosing Institute; Szigeti et al., eLife, 2021; Marschall et al., Journal of Psychopharmacology, 2022; Prochazkova et al., Neuropharmacology, 2026. Updated 3 October 2026 by the Mycotrop team.
Frequently asked questions
How many truffles make a microdose?
About 0.25–1 g of fresh truffles, or 5–10% of a normal dose. Start with half a portion (a quarter with the Mycotrop variety) and only increase if you feel nothing.
Does microdosing work?
That has not been shown. Placebo-controlled studies find little or no difference, and much of the perceived effect may be a placebo effect.
Which pack should I start with?
Mexicana, the mildest variety (2 out of 5), or Tampanensis or Atlantis (3 out of 5). The Mycotrop pack is strong: split its portions into quarters.
How should I store a pack?
In the fridge at 2–4 °C, in its original packaging. Do not freeze the truffles, and throw away any mouldy portion.